The SH-SY5Y neuroblastoma cell line (n. 94030304, Merck, Darmstadt, Germany), derived from a four-year-old, female children was selected as a neuronal model for HSV-1 infection, since the susceptibility of these cells to HSV-1 infection was previously observed [15 (link),16 (link)].
The cells were maintained in 44.5% MEM and 44.5% Ham’s F12, supplemented with 10% fetal bovine serum, non-essential amino acid (10 mM for each amino acid), 2 mM glutamine and 100 U/mL penicillin/streptomycin (all the reagents were from Euroclone, Pero, Milan, Italy). A total of 104 cells were seeded each day prior to infection in 96-multiwell plates (Sarstedt, Munich, Germany) in 100 μL of final volume. After 24 h they were infected with different quantities of viral DNA copies of HSV-1, i.e., 5 × 106, 5 × 105, 5 × 104, 5 × 103, and incubated for 24 h or for 48 h at 37 °C and 5% CO2.
The cells were maintained in 44.5% MEM and 44.5% Ham’s F12, supplemented with 10% fetal bovine serum, non-essential amino acid (10 mM for each amino acid), 2 mM glutamine and 100 U/mL penicillin/streptomycin (all the reagents were from Euroclone, Pero, Milan, Italy). A total of 104 cells were seeded each day prior to infection in 96-multiwell plates (Sarstedt, Munich, Germany) in 100 μL of final volume. After 24 h they were infected with different quantities of viral DNA copies of HSV-1, i.e., 5 × 106, 5 × 105, 5 × 104, 5 × 103, and incubated for 24 h or for 48 h at 37 °C and 5% CO2.
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