Microscope glass slides (50 mm × 75 mm) were used as substrates for fingermark deposition. To degrade any possible extraneous DNA, previous to the experiment, each microscope slide was cleaned with 3% bleach solution, rinsed with bi-distilled water and absolute ethanol, and, before use, irradiated under ultraviolet (UV) light overnight in order to ensure no contaminating DNA was present. The contact with the glass surface was made by the volunteer by pressing palm down the three fingers of each hand for 15 s, exerting pressure but without rubbing.
Touch DNA deposits from the dominant and non-dominant hands were collected according to the following protocol, as represented in Fig.1 . Volunteers were asked to wash their hands with hand soap and water and to dry them in air. During the time interval between handwashing and fingerprint deposition, they were asked to refrain from washing or sanitizing their hands with alcohol, otherwise proceeding with their daily routine.![]()
Touch DNA deposits from the dominant and non-dominant hands were collected according to the following protocol, as represented in Fig.
Schematic diagram of the experimental design regarding the process and timing of DNA fingerprint deposition
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