Adipocyte Differentiation Culture Protocol

The following were obtained from the respective suppliers: Dulbecco’s modified Eagle medium containing 25 mM HEPES, penicillin G potassium salt, streptomycin sulfate, dexamethasone, fatty acid-free bovine serum albumin, and recombinant human insulin (Sigma-Aldrich Corp., St. Louis, MO, USA); L-ascorbic acid phosphate magnesium salt n-hydrate, 3-isobutyl-1-methylxanthine (IBMX), and Triglyceride E-Test Kits (Wako Pure Chemical Industries Ltd., Osaka, Japan); fetal bovine serum (FBS) (MP Biomedicals, Solon, OH, USA); PGD₂, 11d-11m-PGD₂, MRE-269, BW245C, and 15R-15-methyl-PGD₂ (15R-15m-PGD₂) (Cayman Chemical (Ann Arbor, MI, USA); M-MLV reverse transcriptase (Ribonuclease H minus, point mutant) and polymerase chain reaction (PCR) Master Mix (Promega Corp., Madison, WI, USA).

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Nartey M.N., Jisaka M., Syeda P.K., Nishimura K., Shimizu H, & Yokota K. (2023). Prostaglandin D2 Added during the Differentiation of 3T3-L1 Cells Suppresses Adipogenesis via Dysfunction of D-Prostanoid Receptor P1 and P2. Life, 13(2), 370.

Publication 2023

fatty acid-free bovine serum albumin recombinant human insulin L-ascorbic acid phosphate magnesium salt n-hydrate 3-isobutyl-1-methylxanthine Triglyceride E-Test Kits MRE-269 BW245C M-MLV reverse transcriptase PCR) Master Mix

3 isobutyl 1 methylxanthine Bovine serum albumin Cayman Dexamethasone Eagle Fatty acid Fetal bovine serum Hepes Human Insulin L ascorbic acid Magnesium hydrate Mre 269 Penicillin g potassium Pgd2 Phosphate Polymerase chain reaction Promega Reverse transcriptase Ribonuclease h Salt Solon Streptomycin sulfate Triglyceride

Corresponding Organization : Shimane University

Other organizations : Council for Scientific and Industrial Research

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