Total IgE was measured using an indirect enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Chemux Bioscience, South San Francisco, CA, USA). Total IgE serum levels up to 150 IU/mL were considered normal.
The serum aeroallergen-specific IgE for 13 common aeroallergens (Dermatophagoides farinae, Dermatophagoides pteronyssinus, A. alternata, A. niger, A. fumigatus, P notatum, C. albicans, mixed grasses, birch, cat epithelium, dog epithelium, feather mixture, and cockroach) was analyzed by immunoblot assay according to the manufacturer company protocol (AllergyScreen® system, MEDIWISS Analytic GmbH, Moers, Germany). Serum-specific IgE was analyzed by a rapid scanner (Improvio Scanner System, Moers, Germany). The test was considered valid if the process control on each strip’s first position was colored. Specific IgE level ≥ 0.35 IU/mL was deemed positive.
The serum aeroallergen-specific IgE for 13 common aeroallergens (Dermatophagoides farinae, Dermatophagoides pteronyssinus, A. alternata, A. niger, A. fumigatus, P notatum, C. albicans, mixed grasses, birch, cat epithelium, dog epithelium, feather mixture, and cockroach) was analyzed by immunoblot assay according to the manufacturer company protocol (AllergyScreen® system, MEDIWISS Analytic GmbH, Moers, Germany). Serum-specific IgE was analyzed by a rapid scanner (Improvio Scanner System, Moers, Germany). The test was considered valid if the process control on each strip’s first position was colored. Specific IgE level ≥ 0.35 IU/mL was deemed positive.
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