Immunodeficient female non-obese diabetic/severe combined immunodeficiency (NOD-SCID) mice, 8–12 weeks old, were purchased from The Jackson Laboratory (NOD.CB17-Prkdcscid/J; stock # 001303; Bar Harbor, ME). Mice were injected on day 0 with 2F7 cells (106) by intra-peritoneal (i.p) injection as described 17 (link). Mice were treated by i.p. injection with 200 μg of ch128.1, 200 μg of isotype control (IgG3), or buffer alone [Hank’s balanced salt solution (HBSS)] on days 1, 8, and 22. Animals were monitored for tumor development and euthanized when moribund or when the abdomen was distended. Survival was recorded as the number of days from tumor challenge to euthanasia. Survival plots were generated and statistical analysis (log-rank test) was performed using GraphPad Prism Version 5 (GraphPad Software, Inc., La Jolla, CA).
Protocol detail
Immunodeficient Mice for Tumor Development
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Abdomen
Animals
Buffer
Cells
Euthanasia
Female
Igg3
Immunodeficient
Intra peritoneal injection
Isotype
Mice
Non obese diabetic mice
Prism
Salt
Severe combined immunodeficiency
Tumor
Corresponding Organization : University of California, Los Angeles
Other organizations : Beijing Obstetrics and Gynecology Hospital
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Variable analysis
independent variables
- Treatment group (ch128.1, isotype control (IgG3), or buffer alone [Hank's balanced salt solution (HBSS)])
- Tumor development
- Survival (number of days from tumor challenge to euthanasia)
- Immunodeficient female non-obese diabetic/severe combined immunodeficiency (NOD-SCID) mice, 8–12 weeks old
- 2F7 cells (10^6) injected on day 0 by intra-peritoneal (i.p) injection
- Isotype control (IgG3)
- Buffer alone [Hank's balanced salt solution (HBSS)]
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