HE and Nissl staining were carried out according to the standard protocols. A high-power optical microscope (magnification, ×400) was used to observe the pyramidal neurons in the hippocampal CA1 region. Three randomly selected visual fields were used to indicate the histological changes (22 (link)).
TUNEL staining was performed using an In Situ Cell Death Detection Kit, TUNEL red (Vazyme Biotech Co., Jiangsu, China), and cell nuclei were visualized with 4', 6-diamidino-2-phenylindole (DAPI) staining (Vector, Burlingame, CA, USA). TUNEL-positive cells were detected on 10 randomly selected fields from each tissue. The average number of TUNEL-positive cells in the hippocampal CA1 region per high power field was expressed as apoptosis degree (23 (link)).
TUNEL staining was performed using an In Situ Cell Death Detection Kit, TUNEL red (Vazyme Biotech Co., Jiangsu, China), and cell nuclei were visualized with 4', 6-diamidino-2-phenylindole (DAPI) staining (Vector, Burlingame, CA, USA). TUNEL-positive cells were detected on 10 randomly selected fields from each tissue. The average number of TUNEL-positive cells in the hippocampal CA1 region per high power field was expressed as apoptosis degree (23 (link)).
