All odorants (amyl acetate: Aa, ethyl butyrate: Eb, hexanone: Heb) were from Sigma-Aldrich. As odorant stimuli, we used the following mixtures: amyl acetate/air 60%/40%, ethyl butyrate/air 60%/40%, 3-hexanone 60%/40%, amyl acetate/ethyl butyrate 60%/40% and 40%/60%, 3-hexanone/ethyl butyrate 60%/40% and 40%/60%. Each stimulus was repeated 9 and 5 times for anesthetized and awake mice datasets respectively. To test the impact of the number of repetitions for each stimulus (see below), we acquired another dataset and used 8 different stimuli, each applied individually 20 times (Fig. S1 ). All are monomolecular odorants evoking different percept, at least in Humans: amyl acetate, methyl benzoate, ethyl butyrate, geraniol, carvone−(+), carvone−(−), octanal, 3-hexanone.
Four milliliters of pure monomolecular odorant were placed in glass vials. Odorants were delivered for 2 seconds through a custom made olfactometer as described previously [22] (link), [57] (link). The odorant onset was set to arrive during an animal's expiration. An air flow passed through the vials containing the odorants and was further diluted 20 times with clean dry air before being sent to the nose. All mixtures were performed by gas mixing, varying the relative flow of independent stream of odorized air. Because odors were delivered ∼1 cm away from the animal's nose, these values overestimate concentrations actually reaching the nasal cavity. The total flow was constant (0.4 l/min). To maintain a stable odor concentration during the entire stimulus application, we ensured that flows were stationary with a 5 s preloading before the odorant was delivered.
Four milliliters of pure monomolecular odorant were placed in glass vials. Odorants were delivered for 2 seconds through a custom made olfactometer as described previously [22] (link), [57] (link). The odorant onset was set to arrive during an animal's expiration. An air flow passed through the vials containing the odorants and was further diluted 20 times with clean dry air before being sent to the nose. All mixtures were performed by gas mixing, varying the relative flow of independent stream of odorized air. Because odors were delivered ∼1 cm away from the animal's nose, these values overestimate concentrations actually reaching the nasal cavity. The total flow was constant (0.4 l/min). To maintain a stable odor concentration during the entire stimulus application, we ensured that flows were stationary with a 5 s preloading before the odorant was delivered.
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