KPC mice with 3–6 mm tumors were injected with purified anti-CSF1R (anti-CD115, clone AFS98)(18 (link)) derived from a hybridoma generously provided by Dr. Miriam Merad (Icahn School of Medicine at Mount Sinai, New York, NY). Mice received 1 mg/mouse IP on day −3 and day 0 (e.g., day of T-cell therapy), followed by bi-weekly injections of 0.5 mg/mouse IP for 8 or 28 days. A separate cohort of KPC mice was treated once with 100 μg agonistic anti-CD40 (FGK145 hybridoma kindly provided by Dr. Stephen Schoenberger, La Jolla Institute for Allergy and Immunology, San Diego, CA) or isotype control (Rat IgG2a, 2A3; Bioxcell). Anti-CSF-1R and anti-CD40 were purified from culture supernatants of hybridomas grown in a CELLine Flask (BD) in serum-free Hybridoma medium (Sigma) or purchased from BioXcell.
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Anti-CSF1R and Anti-CD40 Therapy for KPC Mice
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Variable analysis
independent variables
- Anti-CSF1R (anti-CD115, clone AFS98) treatment
- Agonistic anti-CD40 (FGK145) treatment
- Isotype control (Rat IgG2a, 2A3) treatment
- Tumor growth and response to treatments
- KPC mice with 3-6 mm tumors
- Tumor size at the start of the experiment
- Positive control: Agonistic anti-CD40 (FGK145) treatment
- Negative control: Isotype control (Rat IgG2a, 2A3) treatment
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