Immunohistochemistry (IHC) and immunofluorescence (IF) were performed at the MSK Molecular Cytology Core Facility using Discovery XT processor (Ventana Medical Systems) as described in (2 (link)). Tumor samples were fixed and embedded in paraffin. Anti-human CD45, anti-Myeloperoxidase, anti-mouse CD31 and anti-mouse CD68 were used, which was followed by biotinylated secondary antibody. The detection was performed using a DAB detection kit (Ventana Medical Systems) or Alexa Fluor™ 488 or 568 Tyramide Reagent (Invitrogen). IHC images were captured from tumor sections using a Nikon ECLIPSE Ni-U microscope and NIS-Elements 4.0 imaging software. IF images were captured with Leica Inverted Confocal SP8 and processed with Imaris (Bitplane). Cells were counted with Qupath 0.1.2.