CytoD was injected laterally at mid-late cellularization with 0.5 mg/ml CytoD in 10 % DMSO (Calbiochem). Double stranded RNA against snail and twist (2 mg/ml) were injected laterally into freshly laid eggs that were incubated 2.5–3 hours before imaging gastrulation.
Protocol detail
Disrupting Gastrulation via CytoD and RNAi
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Corresponding Organization :
Other organizations : Howard Hughes Medical Institute, Institute for Integrative and Experimental Genomics
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Protocol cited in 35 other protocols
Variable analysis
independent variables
- CytoD injection
- Double stranded RNA against snail and twist
- Gastrulation
- DMSO (10%)
- Incubation time (2.5-3 hours)
- Positive control: Not mentioned
- Negative control: Not mentioned
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