T cells or MDMs were infected with HIVLuc-V at 100 ng/mL p24, HIVNL4.3 at 1000 TCID50/106 cells, or HIVAD8 at 5000 TCID50/106 cells.12 (link) HIVLuc–V-infected cells were cultured for 48 hours, and then viral infection was quantified using the Bright-Glo Luciferase kit (Promega, Madison, WI).18 (link) HIV replication in T cells and MDMs was monitored on 7 and 14 days after infection, respectively, using a p24 antigen enzyme-linked immunosorbent assay (ELISA) kit (Perkin Elmer, Boston, MA).14 (link)
Protocol detail
HIV Infection, Replication Quantification
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Corresponding Organization :
Other organizations : Frederick National Laboratory for Cancer Research, United States Department of Agriculture, Covance (United States)
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Variable analysis
independent variables
- HIVLuc-V at 100 ng/mL p24
- HIVNL4.3 at 1000 TCID50/10^6 cells
- HIVAD8 at 5000 TCID50/10^6 cells
- Viral infection quantified using the Bright-Glo Luciferase kit (for HIVLuc-V-infected cells)
- HIV replication monitored using a p24 antigen enzyme-linked immunosorbent assay (ELISA) kit (for T cells and MDMs)
- T cells or MDMs used as the cell types
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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