The modified constant speed rotarod assay was performed as previously described.29 (link), 36 (link) After a training period when each mouse attained stable baseline levels of performance staying at 15 r.p.m. for 60 s, mice received several trials at 15, 25, 35 and 44 r.p.m. rotation speed with 60-s maximum trial length and 5-min intervals between individual trials. Two maximal values per speed per day were used to calculate the average, which was used for subsequent statistical analyses.
For the open field test, mice were placed individually into an open arena and monitored for 5 min via video camera. The resulting data were analyzed using the image processing system EthoVision 3.0 (Noldus Information Technology, Wageningen, Netherlands) and Any-maze 4.82 (Stoelting Co., Wood Dale, IL, USA). For each sample, the system recorded position, object area and the status of defined events to calculate the traveled distance.
To follow the effects of L-3,4-dihydroxyphenylalanine (l -DOPA) on locomotor performance, 17 weeks post Tam (wpT) injection control and Dicerfl/fl/DATCreERT2 mice were tested in the modified rotarod assay before and immediately after an intraperitoneal injection of 20 mg/kg l -DOPA (Sigma-Aldrich) complemented with 12 mg/kg benserazide (Sigma-Aldrich).
For the open field test, mice were placed individually into an open arena and monitored for 5 min via video camera. The resulting data were analyzed using the image processing system EthoVision 3.0 (Noldus Information Technology, Wageningen, Netherlands) and Any-maze 4.82 (Stoelting Co., Wood Dale, IL, USA). For each sample, the system recorded position, object area and the status of defined events to calculate the traveled distance.
To follow the effects of L-3,4-dihydroxyphenylalanine (
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