Whole-Body Gamma Irradiation and Bone Marrow Transplantation

Bone marrow transplantation was performed as described previous.^{60 (link)} In brief, WT mice at 6 weeks of age underwent whole-body gamma irradiation with 137Cs using a Gammacell 40 irradiator (MDS Nordion, Ontario, Canada). A lead shield was used to protect the head and eyes from radiation. A dose of 8.5 Gy (850 rads) was used to ablate the marrow. Within 24 h after irradiation, mice received bone marrow transplant from 6-week-old GFP transgenic mice through tail vein as a 200 μl cell suspension containing 0.7–1.0 × 10⁷ cells. 6 weeks later, mice were subjected to IR.

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Ha Y., Liu H., Xu Z., Yokota H., Narayanan S.P., Lemtalsi T., Smith S.B., Caldwell R.W., Caldwell R.B, & Zhang W. (2015). Endoplasmic reticulum stress-regulated CXCR3 pathway mediates inflammation and neuronal injury in acute glaucoma. Cell Death & Disease, 6(10), e1900-.

Publication 2015

Gammacell 40 irradiator

Bone marrow transplant Cells Eyes Gamma Head Irradiation Marrow Mice Radiation Rads Transgenic mice Vein Whole body irradiation

Corresponding Organization : The University of Texas Medical Branch at Galveston

Other organizations : Georgia Regents Medical Center, Augusta University

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Variable analysis

independent variables

Whole-body gamma irradiation with 137Cs using a Gammacell 40 irradiator
Dose of 8.5 Gy (850 rads) to ablate the marrow
Bone marrow transplant from 6-week-old GFP transgenic mice

dependent variables

Outcome after 6 weeks of bone marrow transplantation

control variables

Age of mice (6 weeks)
Use of a lead shield to protect the head and eyes from radiation

positive controls

Not specified

negative controls

Not specified

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