Detecting DNA Damage in H. pylori

To detect DNA damage in H. pylori [44 (link)], a TransDetect In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit (Transgen Biotech, Beijing, China) was employed. Accuri C6 (BD, Franklin Lakes, Germany) flow cytometer and LSM510 confocal (Zeiss, Oberkochen, Germany) were used to detect the fluorescence signal changes. All flow cytometry data were collected using the Accuri C6 software. At least 10,000 cells were collected and analyzed for each sample. Flow data were processed and analyzed with CFlow Plus (BD, Franklin Lakes, Germany).

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Wang G., Pang J., Hu X., Nie T., Lu X., Li X., Wang X., Lu Y., Yang X., Jiang J., Li C., Xiong Y.Q, & You X. (2019). Daphnetin: A Novel Anti-Helicobacter pylori Agent. International Journal of Molecular Sciences, 20(4), 850.

Publication 2019

TransDetect In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit Accuri C6 CFlow Plus

Apoptosis Cells Dna damage Flow cytometry Fluorescein Fluorescence H pylori Tunel

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Fluorescence signal changes

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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