Wheat Protein Identification by MALDI-TOF/TOF-MS

The DAP spots from 2-DE gels were excised and digested with trypsin in 2 mL centrifuge tubes as described by Lv et al. [92 (link)] After bleaching and drying, the tryptic peptides analysis was performed by ABI 4800 Proteomic Analyzer matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) instrument (Applied Biosystems/MDS Sciex, USA). The MS/MS spectra were searched against the NCBI wheat protein database (136,744 entries)-the nonredundant Ensembl plants database of 2021 using software MASCOT version 2.1 (Matrix Science), and the related parameter settings were according to Cao et al. [90 (link)].

Free full text: Click here

Lu F., Duan W., Cui Y., Zhang J., Zhu D., Zhang M, & Yan Y. (2022). 2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress. BMC Genomics, 23, 369.

Publication 2022

ABI 4800 Proteomic Analyzer MASCOT version 2.1

Gels Maldi ms Mass spectrometry Ms ms Peptides Plants Spots Tryptic Wheat

Corresponding Organization : Heze University

Other organizations : Capital Normal University

Top 5 similar protocols

Variable analysis

independent variables

Trypsin digestion of DAP spots from 2-DE gels

dependent variables

Tryptic peptides analysis by MALDI-TOF/TOF-MS

control variables

Trypsin digestion protocol described by Lv et al. [92]
MASCOT version 2.1 software with related parameter settings according to Cao et al. [90]

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!