During RHC, the fasting blood samples taken from inferior vena cava were collected in EDTA-tubes, centrifuged at 3500× g for 15 min, aliquoted and stored in −80 °C in the Biobank Unit, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia, until assayed for bio-ADM. An aliquoted sample was thawed, and bio-ADM was measured using a chemiluminescence immunoassay (sphingotest® bio-ADM®, SphingoTec GmbH, Hennigsdorf, Germany) as previously described [6 (link)].
The hemoglobin concentration, hematocrit percentage and N terminal-pro brain natriuretic peptide (NT-proBNP) level (electrochemiluminescence immunoassay (ElecsysProBNP II) and a Cobas e immunoassay analyzer (Roche Diagnostics, Germany)) were measured using blood samples taken during RHC at index of diagnosis in Dr. Sardjito Hospital central laboratory, as previously described [13 (link)].
The hemoglobin concentration, hematocrit percentage and N terminal-pro brain natriuretic peptide (NT-proBNP) level (electrochemiluminescence immunoassay (ElecsysProBNP II) and a Cobas e immunoassay analyzer (Roche Diagnostics, Germany)) were measured using blood samples taken during RHC at index of diagnosis in Dr. Sardjito Hospital central laboratory, as previously described [13 (link)].
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