Evaluating Chemotaxis and Cell Motility

Cell motility parameters of maximum Euclidean and Accumulated distances and COM vector resolution in the Y-axis direction for each cell tracked over 48 hrs, were resolved via sequential use of Nd-to-Image6d (National Institutes of Health, Bethesda, USA), Manual Tracking (Fabrice Cordelières, Institut Curie, Orsay, France), and Chemotaxis and Migration Tool 2.0 (ibidi, Munich, Germany) plug-ins, all running on an ImageJ platform as previously described97 (link). COM is a strong parameter for evaluating chemotaxis, and measures the spatial average displacement of all cell endpoints with positive or negative coordinates, depending on the direction of movement of a single cell or a population of cells. COM displacement in the Y-axis further specifies directionality of cell movement towards the SRR. Cell tracking data selected for analysis included only video recordings of RPC and PPC movement between 18 and 42 hrs to account for complete 24 hr periods of sustained steady-state gradients of SDF-1α within the microchannel. A two-sample Student’s t-test (t) assuming unequal variances was performed to assess significant difference in mean motility parameters between control and test groups.

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Unachukwu U.J., Warren A., Li Z., Mishra S., Zhou J., Sauane M., Lim H., Vazquez M, & Redenti S. (2016). Predicted molecular signaling guiding photoreceptor cell migration following transplantation into damaged retina. Scientific Reports, 6, 22392.

Publication 2016

Chemotaxis and Migration Tool 2.0

Axis Cells Chemotaxis Motility Movement Student Vector

Corresponding Organization :

Other organizations : The Graduate Center, CUNY, City University of New York, Lehman College, City College of New York, Hunter College

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Variable analysis

independent variables

Cell type (RPC and PPC)

dependent variables

Maximum Euclidean distance
Accumulated distance
COM vector resolution in the Y-axis direction

control variables

Time period of cell tracking (18-42 hrs)
Sustained steady-state gradients of SDF-1α within the microchannel

controls

Control group
Test group

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