Protocol detail

Molecular Biology Reagent Verification

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All DNA constructs were made in the corresponding author’s laboratory and verified by DNA sequencing and/or restriction enzyme analysis. Chemicals were purchased at molecular biology or analytical grade purity from established vendors (e.g., Sigma-Aldrich, VWR). Enzymes were obtained from Promega or New England Biolabs. The following antibodies were used for western blotting or immunoprecipitation: anti-Flag M2 (Sigma-Aldrich F1804), anti-Myc 9E10 (Sigma-Aldrich M4439), anti-cyclin D1 DCS6 (Cell Signaling #2926), anti-SMAD4 B-8 (Santa Cruz sc-7966) and anti-actin (GenScript A00730) mouse monoclonal antibodies; anti-ETV1 (Abcam ab81086 or our previously described #959 antibody^{9 (link)}), anti-p21 H-164 (Santa Cruz sc-756), anti-PMEPA1 2A12 (Abnova H00056937-M01) and anti-SMAD3 (Zymed 51–1500) rabbit polyclonal antibodies; and anti-p-SMAD3-Ser423/425 C25A9 (Cell Signaling #9520) rabbit monoclonal antibodies.

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Oh S., Shin S., Song H., Grande J.P, & Janknecht R. (2019). Relationship between ETS Transcription Factor ETV1 and TGF-β-regulated SMAD Proteins in Prostate Cancer. Scientific Reports, 9, 8186.

Publication 2019

anti-Flag M2 anti-Myc 9E10 anti-SMAD4 B-8 anti-actin anti-ETV1 anti-p21 H-164

Actin Antibodies Cyclin d1 Dna constructs Enzymes Immunoprecipitation Monoclonal antibodies Mouse Promega Rabbit Restriction enzyme analysis Smad3 Smad4

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels measured by western blotting or immunoprecipitation

control variables

DNA constructs were made in the corresponding author's laboratory and verified by DNA sequencing and/or restriction enzyme analysis
Chemicals were purchased at molecular biology or analytical grade purity from established vendors (e.g., Sigma-Aldrich, VWR)
Enzymes were obtained from Promega or New England Biolabs

controls

Positive controls: None explicitly mentioned
Negative controls: None explicitly mentioned

Annotations

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