HPLC-ESI-MS Analyses of Flavonoids

For HPLC-ESI-MS analyses of the flavonoids, we followed the method of Yi et al. (2016 (link)) with modification. Experimental standards of cyanidin-3-O-galactoside, quercetin-3-O-glucoside, astilbin, phloridzin, rutin, quercetin-3-O-arabinoside, quercetin, and luteolin were purchased from Sigma-Aldrich (Steinheim, Germany); experimental standards of 4-O-coumaroyl quinic acid, procyanidin B2, (-)-epicatechin, and taxifolin-3-O-glucoside were purchased from Sigma-Aldrich (Poole, U.K.); data obtained for experimental standards and information regarding the identified compounds were combined to identify the flavonoids.

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Qin X., Lu Y., Peng Z., Fan S, & Yao Y. (2018). Systematic Chemical Analysis Approach Reveals Superior Antioxidant Capacity via the Synergistic Effect of Flavonoid Compounds in Red Vegetative Tissues. Frontiers in Chemistry, 6, 9.

Publication 2018

cyanidin-3-O-galactoside quercetin-3-O-glucoside astilbin phloridzin rutin quercetin-3-O-arabinoside quercetin luteolin procyanidin B2 (-)-epicatechin

Astilbin Cyanidin 3 galactoside Epicatechin Flavonoids Glucoside Hplc Luteolin Phloridzin Procyanidin b2 Quercetin Quercetin 3 o glucoside Quinic acid Rutin Taxifolin

Corresponding Organization : Beijing University of Agriculture

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Variable analysis

independent variables

Method of Yi et al. (2016) with modification

dependent variables

Identification of flavonoids

control variables

Experimental standards of cyanidin-3-O-galactoside, quercetin-3-O-glucoside, astilbin, phloridzin, rutin, quercetin-3-O-arabinoside, quercetin, and luteolin purchased from Sigma-Aldrich (Steinheim, Germany)
Experimental standards of 4-O-coumaroyl quinic acid, procyanidin B2, (-)-epicatechin, and taxifolin-3-O-glucoside purchased from Sigma-Aldrich (Poole, U.K.)

positive controls

Experimental standards of cyanidin-3-O-galactoside, quercetin-3-O-glucoside, astilbin, phloridzin, rutin, quercetin-3-O-arabinoside, quercetin, and luteolin
Experimental standards of 4-O-coumaroyl quinic acid, procyanidin B2, (-)-epicatechin, and taxifolin-3-O-glucoside

negative controls

Not specified

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