MZptk7, MZvangl2 or Xdd1mRNA-injected cells were labelled with rhodamine dextran (red 10,000 MW, Sigma) by injections at the one-cell stage. Labelled cells were transplanted from sphere (4 hpf) stage embryos into the ventral margin of unlabelled WT hosts at shield stage (6 hpf) as previously described38 (link). Embryos were screened at 24 hpf on an Axio Imager.M1 (Zeiss) compound microscope and embryos containing significant rhodamine-labelled cells (mutant/Xdd1 cells) in the embryonic tail were grown to larval stages.
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Cell Transplantation in Zebrafish Embryos
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Corresponding Organization :
Other organizations : Hospital for Sick Children, Texas Scottish Rite Hospital for Children, University of Toronto
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Variable analysis
independent variables
- MZptk7
- MZvangl2
- Xdd1mRNA
- Presence and distribution of rhodamine dextran-labeled cells (mutant/Xdd1 cells) in the embryonic tail at 24 hpf
- Unlabeled wild-type (WT) host embryos
- Positive control: Transplantation of rhodamine dextran-labeled cells into the ventral margin of unlabeled WT host embryos at shield stage (6 hpf)
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