Cells from the control group, hsa-miR-9-5p agomir group and NC group were
treated with different doses of X-rays (0, 2, 4, 6 and 8 Gy) and then
dispensed into 96-well plates (1×104 cells/ well) in
triplicates. The plates were incubated at 37°C, 5% CO2 for
48 hours. Then 10µl CCK-8 (Sangon, Shanghai, China) was added to each
well for an additional 4 hours. The absorbance was obtained at 490 nm
using a microplate reader (BioTek, Winooski, VT, USA).10 (link)