Peptide Synthesis Protocol

Fmoc-protected amino acids, N,N’-diisopropylcarbodiimide, Oxyma Pure and trifluoroacetic acid were purchased from Iris Biotech (Marktredwitz, Germany). Cl-MPA ProTide Resin was supplied by CEM (Matthews, NC, USA). Dess-Martin Periodinane was supplied by Fluorochem (Hadfield, UK). Reagents for electrophoresis were purchased from Biorad (Hercules, CA, USA). The cell culture reagents, if not otherwise stated, were purchased from Merck (Darmstadt, Germany). All general purpose solvents (dimethylformamide, dichloromethane, dimethyl sulfoxide) as well as HEPES and Tris were supplied by Fisher Scientific (Waltman, MA, USA). Acetonitrile (Supelco) was purchased from Merck (Darmstadt, Germany).