IHC was performed with an UltraSensitive SP Kit (Maixin-Bio, Fujian, China) following the manufacturer’s instructions as previously described41 (link),42 (link). Cells were incubated with a primary antibody against ZNF382 (1:50 dilution, Sigma-ldrich) overnight at 4 °C and then incubated with a secondary antibody at 37 °C for 30 min. All IHC images were captured under a microscope at ×400 magnification.
Free full text: Click here