Cell Culture Protocols for Cancer Research

NIH3T3-E1 fibroblasts were obtained from ATCC (Manassas, VA, USA) and cultured as previously described [25 (link),26 (link)]. The human lung cancer cell lines A549 and H2030 were obtained from ATCC and cultured in RPMI-1640 medium containing 10% FBS and antibiotics as previously described [23 (link)]. CAPAN-1 human pancreatic adenocarcinoma cell line was purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS and antibiotics as previously described [23 (link)]. Sufu-/- mouse embryonic fibroblasts were a gift from Dr. Matthew Scott of Stanford University. TGFβRI-deficient mink lung epithelial cells, R1BL17, were maintained in MEM containing 10% FBS and non-essential amino acids as described [27 (link)]. Gant61, and SB431542 were obtained from Cayman Chemical Company (Ann Arbor, MI, USA), HPI-1 was obtained from Abcam (Cambridge, UK), TGFβ1 was obtained from R&D Systems (Minneapolis, MN, USA). Carboplatin was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).