Spleen Removal in Murine Ischemic Stroke

The mice were anesthetized by inhalation of Isoflurane (Isoflurane, Abbott, Maidenhead, UK; dosages detailed in the MCAO procedure description). The spleen was removed via a dorsolateral approach, the fur around the 13th rib was shaved, the area was disinfected with 70% ethanol, and an incision was made below the 13th rib to access the spleen. It was separated from its blood vessels using electrocautery, and the wound was closed with metal clips. The animals were monitored for one day in a heated cage with analgesia using 0.1 mL of buprenorphine (Buprenovet, Bayer, Leverkusen, Germany). Full recovery from the operation was deemed after a week (22 (link),23 (link)).

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Romić D., Berecki M., Srakočić S., Josić Dominović P., Justić H., Hamer D., Petrinec D., Radmilović M., Hackenberger B., Gajović S, & Glasnović A. (2024). Innovative statistical approaches: the use of neural networks reduces the sample size in the splenectomy-MCAO mouse model. Croatian Medical Journal, 65(2), 122-137.

Publication 2024

Isoflurane buprenorphine Buprenovet

Corresponding Organization :

Other organizations : University of Zagreb

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Variable analysis

independent variables

Inhalation of isoflurane (Isoflurane, Abbott, Maidenhead, UK)

dependent variables

Spleen removal via a dorsolateral approach
Time to full recovery from the operation (1 week)

control variables

Dosages of isoflurane (detailed in the MCAO procedure description)
Fur around the 13th rib was shaved
Area was disinfected with 70% ethanol
Incision was made below the 13th rib to access the spleen
Spleen was separated from its blood vessels using electrocautery
Wound was closed with metal clips
Animals were monitored for one day in a heated cage with analgesia using 0.1 mL of buprenorphine (Buprenovet, Bayer, Leverkusen, Germany)

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