Plasmid Constructs for Cell Biology

The following plasmids were used in this study: pMWH₆C-µ2(160-435)50 (link), Myc-µ230 (link), Integrin α2-GFP29 (link), Integrin α4-GFP51 (link), integrin α5-GFP52 (link), dsRed-Rab753 (link), RFP-Rab553 (link), dsRed-Rab2129 (link), pPS-CFP2-αV-Integrin (Addgene, 57212), mCherry-Rab4a (Addgene, 55125), mCherry-Rab11 (Addgene, 55124) Point mutations were done by QuikChange II Mutagenesis kit (Agilent technologies) according to manufacturer’s instructions. All constructs were checked for integrity and correctness with sequencing.

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De Franceschi N., Arjonen A., Elkhatib N., Denessiouk K., Wrobel A.G., Wilson T.A., Pouwels J., Montagnac G., Owen D.J, & Ivaska J. (2016). Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2. Nature structural & molecular biology, 23(2), 172-179.

Publication 2016

mCherry-Rab11 QuikChange II Mutagenesis kit

Integrin Integrin α2 Mutagenesis Plasmids Point mutations αv integrin

Corresponding Organization : University of Turku

Other organizations : Turku Centre for Biotechnology, Institut Gustave Roussy, Åbo Akademi University, University of Cambridge, Inserm

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Variable analysis

independent variables

PMWH6C-μ2(160-435)50
Myc-μ230
Integrin α2-GFP29
Integrin α4-GFP51
Integrin α5-GFP52
DsRed-Rab753
RFP-Rab553
DsRed-Rab2129
PPS-CFP2-αV-Integrin
MCherry-Rab4a
MCherry-Rab11

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

None explicitly mentioned

Annotations

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