bEnd3 cellscultured on 24 well inserts were treated for 48 h with 2.2 μg/mL of Crocus sativus extract or vehicle. Aβ transport was performed as described previously.53 (link) Briefly, treatments were removed and replaced with fresh media for 4 h, as a washout period. The basolateral to apical transport (B → A) was initiated by the addition of media containing 0.1 nM 125I-Aβ40 and 14C-inulin (0.1 μCi/mL) to B side (lower chamber) and media alone to A side (upper chamber) for 30 min. Aliquots from both A and B sides were taken for radioactivity analysis using Wallac1470 Wizard Gamma and Wallac 1414 WinSpectral liquid scintillation counters (PerkinElmer Inc. Waltham, MA).
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Crocus sativus Extract Effect on Aβ Transport
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Other organizations : University of Louisiana at Monroe, Indian Institute of Integrative Medicine
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Variable analysis
independent variables
- Treatment with 2.2 μg/mL of Crocus sativus extract or vehicle
- A-beta transport (basolateral to apical, B → A)
- BEnd3 cells cultured on 24 well inserts
- Washout period of 4 h
- Addition of 0.1 nM 125I-Aβ40 and 14C-inulin (0.1 μCi/mL) to the basolateral side
- Addition of media alone to the apical side
- Measurement of radioactivity in both apical and basolateral sides
- Vehicle treatment
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