FITC-BSA Tracer Perfusion in Lung and Mesentery

FITC-BSA (9mg/kg) was injected via the jugular vein in rats. 1 h after the administration, the abdominal cavity was opened, and the abdominal aorta was cut open, slowly infusing the normal saline solution through the jugular vein until the lung tissue became white. The right-lung tissue was embedded with OCT glue, and a frozen section (section thickness 10–15μm) was performed. Tissues were fixed with 4% paraformaldehyde for 15 min, permeated with 0.1% Triton-X for 5 min, and incubated with DAPI (1:50) at 37°C for 30 min. The FITC-BSA exudation in lung tissue was observed under a laser confocal microscope. A 2 cm incision was made along the middle of the abdomen for other rats to select mesenteric fixation with abundant micro-vessels. FITC-BSA was injected into the femoral vein. After 6 minutes, the leakage of FITC-BSA into mesenteric micro-vessels was observed by an inverted microscope (Hamamatsu, Japan).19 (link)

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She H., Hu Y., Zhou Y., Tan L., Zhu Y., Ma C., Wu Y., Chen W., Wang L., Zhang Z., Wang L., Liu L, & Li T. (2021). Protective Effects of Dexmedetomidine on Sepsis-Induced Vascular Leakage by Alleviating Ferroptosis via Regulating Metabolic Reprogramming. Journal of Inflammation Research, 14, 6765-6782.

Publication 2021

inverted microscope

Abdomen Abdominal aorta Dapi Femoral vein Fitc bsa Frozen section Jugular vein Laser microscope Lung Mesenteric Microscope Normal saline solution Paraformaldehyde Rats Tissue Vessels

Corresponding Organization :

Other organizations : Daping Hospital, Army Medical University

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Variable analysis

independent variables

Injection of FITC-BSA (9mg/kg) via the jugular vein in rats

dependent variables

FITC-BSA exudation in lung tissue observed under a laser confocal microscope
Leakage of FITC-BSA into mesenteric micro-vessels observed by an inverted microscope

control variables

Infusion of normal saline solution through the jugular vein until the lung tissue became white
Fixation of right-lung tissue with 4% paraformaldehyde for 15 min
Permeation of tissues with 0.1% Triton-X for 5 min
Incubation with DAPI (1:50) at 37°C for 30 min
Mesenteric fixation with abundant micro-vessels
Injection of FITC-BSA into the femoral vein after 6 minutes

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