Phylogenetic Analysis of WRKY Proteins

MAFFT version 7 (https://mafft.cbrc.jp/alignment/server/ (accessed on 8 December 2021)) was used to perform multi-sequence alignment on the TKS WRKY protein sequence domain with G-INS-1 (progressive method with an accurate guide tree) settings. The phylogenetic tree was constructed by MEGA software (version 7.0, Mega Limited, Auckland, New Zealand) with the neighbor-joining (NJ) method based on the comparison between TKS and Arabidopsis proteins; then we used Illustrator software (version 2019, Adobe, San Jose, CA, USA) to beautify and add color. The sequences of the WRKY protein from Arabidopsis, maize [50 (link)], rice [61 (link),67 (link)], grape [47 (link)], Brachypodium distachyon [68 (link)], pineapple [64 (link)], peach [69 ], and poplar [36 (link)] were obtained according to the corresponding literature and instructions downloaded from the Phytozome databases (https://phytozome.jgi.doe.gov/ (accessed on 27 December 2021)).

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Cheng Y., Luo J., Li H., Wei F., Zhang Y., Jiang H, & Peng X. (2022). Identification of the WRKY Gene Family and Characterization of Stress-Responsive Genes in Taraxacum kok-saghyz Rodin. International Journal of Molecular Sciences, 23(18), 10270.

Publication 2022

Illustrator software

Arabidopsis Arabidopsis proteins Brachypodium distachyon Grape Maize Peach Pineapple Poplar Protein sequences Rice Sequence alignment Tree

Corresponding Organization : Anhui Agricultural University

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Variable analysis

independent variables

MAFFT version 7 used to perform multi-sequence alignment on the TKS WRKY protein sequence domain with G-INS-1 (progressive method with an accurate guide tree) settings

dependent variables

Phylogenetic tree constructed by MEGA software (version 7.0) with the neighbor-joining (NJ) method based on the comparison between TKS and Arabidopsis proteins

control variables

Sequences of the WRKY protein from Arabidopsis, maize, rice, grape, Brachypodium distachyon, pineapple, peach, and poplar obtained according to the corresponding literature and instructions downloaded from the Phytozome databases

controls

No positive or negative controls specified

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