ATR, ATM, Caspase, and Calpain Inhibitors

WERI-Rb1 cells were treated with ATR inhibitor VE822 (1 μM), ATM serine/threonine kinase (ATM) inhibitor KU55933 (1 μM), Caspase inhibitor Z-VAD-FMK (10 μM), or calpain inhibitor Calpeptin (10 μM), each for 1 h prior to transfection or the UV irradiation. These doses were selected based on dose-response experiments, which demonstrated that at these concentarations, the kinases were effectively inhibited without initiating an apoptotic response.

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Liu J., Tan X., Li L., Cao L., Zhou Y., Li H, & Peng T. (2024). Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage. Cell Death Discovery, 10, 130.

Publication 2024

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, Hubei University of Medicine

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Variable analysis

independent variables

ATR inhibitor VE822 (1 μM)
ATM serine/threonine kinase (ATM) inhibitor KU55933 (1 μM)
Caspase inhibitor Z-VAD-FMK (10 μM)
Calpain inhibitor Calpeptin (10 μM)

dependent variables

Not explicitly mentioned

control variables

Dose-response experiments
Concentrations of inhibitors that effectively inhibit kinases without initiating an apoptotic response

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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