Using A549 cells, we treated TGF-β (Sigma-Aldrich, #T7039). Before the treatment of TGF-β, cells were starved with 0.1% FBS for 24 h43 (link). After starvation, we treated 5 ng/ml TGF-β diluted with 2 mg/ml Bovine Serum Albumin (BSA, Sigma-Aldrich, #A9647) for 48 h with 0.1% FBS. In the case of the combination with TGF-β and Kv3.4 overexpression, Kv3.4-overexpressed cells were also starved with 0.1% FBS with G-418 for 24 h. And then, 2 ng/ml of TGF-β was treated in the cells for 24 h44 (link). After the treatment, the cells were prepared for RNA or protein extraction.
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