Screening Metal-Resistant Lactic Acid Bacteria

Metal solutions, Ni(NO₃)₂, Pb(NO₃)₂, Cd(NO₃)₂ and Cr(NO₃)₂ (Sigma-Aldrich, USA), were sterilized by filtration through 0.45μm Millipore bacterial filters (Advantec, Tokyo, Japan). Metal-resistant profiles of LAB isolates were conducted on MRS agar plates amended with metals concentrations (up to 600 ppm) and incubated under anaerobic conditions at 30 °C for 1–3 days. The minimum inhibitory concentrations (MICs) were assessed by determining the lowest metal concentrations that completely inhibited LAB growth^{39 (link)}. The higher-growth-exhibiting isolates were selected as potential metal-resistant LAB isolates for further studies.

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Ameen F.A., Hamdan A.M, & El-Naggar M.Y. (2020). Assessment of the heavy metal bioremediation efficiency of the novel marine lactic acid bacterium, Lactobacillus plantarum MF042018. Scientific Reports, 10, 314.

Publication 2020

Ni(NO3)2 Pb(NO3)2 Cd(NO3)2

Agar Bacterial Filtration Metal Minimum inhibitory concentrations

Corresponding Organization : Alexandria University

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Variable analysis

independent variables

Metal concentrations (up to 600 ppm)

dependent variables

Minimum inhibitory concentrations (MICs) of metals that completely inhibited LAB growth

control variables

Anaerobic conditions at 30 °C for 1–3 days
MRS agar plates

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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