OCT-embedded frozen muscle was cross-sectioned into 10 µm thick sections, stained with Hematoxylin & Eosin (H&E) or picrosirius red as previously described (Hammers et al., 2020 (link)), and visualized with a Leica DMR bright-field microscope equipped with a digital camera (Leica No. DFC480). Image analysis was performed using FIJI software.
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