The diagnosis of EGFR mutation including exon 20 insertion was performed based on PCR-based methods (therascreen EGFR RGQ PCR Kit [Scorpion-ARMS technology]; QIAGEN, Hilden, Germany, and Cobas EGFR Mutation Test v2; Roche Diagnostics, Basel, Switzerland)21 (link),22 (link) and next-generation sequencing (NGS) testing (OncoGuide NCC Oncopanel System, Sysmex, Kobe, Japan)23 (link).
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