Whole Mount Analysis of Lung Metastases

Whole mount analysis was performed on the lungs in order to identify and enumerate metastases. Briefly, lungs were fixed overnight in 4% paraformaldehyde, stored overnight in 70% ethanol, and stained 48 hrs in carmine aluminum solution (0.22% carmine, 0.55% aluminum potassium sulfate). The lungs were than dehydrated by 70%, 90%, 100% ethanol and xylene respectively. The tumor nodules in lungs were counted by a blinded observer on a dissection microscope.

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Yokdang N., Hatakeyama J., Wald J.H., Simion C., Tellez J.D., Chang D.Z., Swamynathan M.M., Chen M., Murphy W.J., Carraway KL I.I.I, & Sweeney C. (2015). LRIG1 opposes epithelial to mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene, 35(22), 2932-2947.

Publication 2015

Aluminum Aluminum potassium sulfate Carmine Dissection Ethanol Lungs Metastases Microscope Paraformaldehyde Tumor Xylene

Corresponding Organization :

Other organizations : University of California, Davis

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Variable analysis

independent variables

Staining duration: 48 hrs in carmine aluminum solution

dependent variables

Enumeration of tumor nodules in lungs

control variables

Lung tissue fixation: overnight in 4% paraformaldehyde
Lung tissue storage: overnight in 70% ethanol
Dehydration steps: 70%, 90%, 100% ethanol and xylene

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