Cell migration assay was evaluated by using wound healing and Transwell membrane chambers (8 µm pore size; Corning Inc., New York, NY, USA). The wound healing assay was performed as previously described (16 (link)). Briefly, the cells were seeded in 12-well plates and treated with ISO at 90% confluence and normal culture medium was added to the control cells. For Transwell membrane chambers, cells were seeded into the upper chambers and cultured with or without ISO for 36 h. For invasion assay, top chambers coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) at a concentration of 1.37 mg/ml. Upper chambers were fixed in 4% paraformaldehyde at room temperature for 30 min, stained using 0.1% crystal violet at room temperature for 5 min, then counted using a Axioskop 40 (Carl Zeiss AG) microscope.