N. gaditana dry biomass was obtained from AlgaEnergy (Madrid, Spain). The N. gaditana extract was obtained and analyzed in terms of lipid classes and fatty acid profile following previously optimized methods [1 (link)]. SOFTISAN® 649 (bis-diglyceryl Polyacyladipate-2), a lanolin substitute widely use in the industry [43 (link)], was obtained from Sasol Germany GmbH & Co (Witten, Germany). Tween® 80 (polysorbate 80) was purchased from Sigma Aldrich (Madrid, Spain). Soy lecithin was purchased from Acopharma (Barcelona, Spain). Ethyl acetate was from Vaz Pereira S.A. (Sintra, Portugal). MilliQ water was obtained from a MilliQ plus system (Millipore, Germany). Methanol was purchased from Lab Scan Analytical Sciences (Gliwice, Poland). The HPLC-grade solvents (2,2,4-trimethyl pentane, methyl tert-butyl ether (MTBE)) were purchased from Macron Fine Chemicals (Gliwice, Poland). Absolute ethanol (PRS grade), sodium hydrogen carbonate, and potassium hydroxide were purchased from Panreac Química S.A (Barcelona, Spain). Glyceryl trilinoleate, dioleoylglycerol (mixture of 1,3- and 1,2-isomers), 1-oleoyl-rac-glycerol, phosphatidylethanolamine, mono galactosyl glycerol, di galactosyl glycerol, oleic acid, and ethyl linoleate used as HPLC standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and solvents used were of analytical or HPLC grade.
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