From brains injected with sindbis virus, tissue was sectioned at 200 μm on a Leica 3050S cryostat that had been cleaned with RNAseZap prior to use. Sections were collected over dry ice and stored at −80°C prior to dissection. Cortical areas were then dissected according to sulcal landmarks over dry ice. The areas that were collected and our operational definitions of their boundaries can be found in this table.
Samples from each area were combined across 3 sections in the anterior/posterior plane into 1.5-ml Eppendorf tubes, which were stored at −80°C prior to shipping frozen on dry ice for sequencing.
Samples from each area were combined across 3 sections in the anterior/posterior plane into 1.5-ml Eppendorf tubes, which were stored at −80°C prior to shipping frozen on dry ice for sequencing.
