V. cholerae strains in this study are derivatives of V. cholerae WT El Tor strain N16961 (Heidelberg et al., 2000 (link)). Construction of plasmids and mutant V. cholerae strains is described in the next section along with a table of strains and plasmids used in this work (Supplementary file 4 ).
Strains were grown at 30 or 37°C in Luria-Bertani (LB-Miller, Fisher Bioreagents# BP97235) with or without 1% NaCl, 1% sucrose, or 10% sucrose, or in M9 minimal media + 0.4% glucose (Cold Spring Harbor Protocols) where indicated in the figure legends. Growth media were supplemented with kanamycin (50 μg/mL), ampicillin (25 μg/mL), or chloramphenicol (5 μg/mL) in plates and overnight cultures when needed to maintain plasmids or chromosomal integration of suicide vectors. Genes under Para and Ptac regulation were induced with 0.4% L-arabinose or 200 μM isopropyl-β-D-1-thiolgalactopyranoside (IPTG), respectively.
Strains were grown at 30 or 37°C in Luria-Bertani (LB-Miller, Fisher Bioreagents# BP97235) with or without 1% NaCl, 1% sucrose, or 10% sucrose, or in M9 minimal media + 0.4% glucose (Cold Spring Harbor Protocols) where indicated in the figure legends. Growth media were supplemented with kanamycin (50 μg/mL), ampicillin (25 μg/mL), or chloramphenicol (5 μg/mL) in plates and overnight cultures when needed to maintain plasmids or chromosomal integration of suicide vectors. Genes under Para and Ptac regulation were induced with 0.4% L-arabinose or 200 μM isopropyl-β-D-1-thiolgalactopyranoside (IPTG), respectively.
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