A Sonovitro ultrasonic irradiator instrument (ShengXiang, China) was used for US treatment. Ultrasound scanning system (SonoKang, China) and High-resolution small animal ultrasound imaging system (S-Sharp, United States) for US imaging in vitro or in vivo, respectively. The ultrasound signals were recorded before and after the ultrasound treatment.
For in vitro US imaging, the effect of ultrasound intensity on ultrasound imaging of SonoBacteriaBot has been studied, DOX-PFP-PLGA@EcM (1 mL, 1.25 mg/mL) placed in a gel model, were exposed to US irradiation with different intensities (1–3 W/cm2, 1 min, 1 MHz), and then captured US imaging (MI = 0.7, 7.5 MHz). To evaluate the effect of ultrasound treatment time on the imaging effect of SonoBacteriaBot in vitro. DOX-PFP-PLGA@EcM was subjected to ultrasonic irradiation of different durations (0–10 min, 1 W/cm2, 1 MHz), and captured US imaging. Then, Imaging of DOX-PFP-PLGA@EcM after phase transformation by ultrasound treatment under an ultrasound intensity of 1 W/cm2 for 2 min, was observed continuously (0–10 min). Ultrasound images were acquired at different times to evaluate the duration of the imaging signal.
For in vivo US imaging, mice were intravenously injected with DOX-PFP-PLGA @EcM in PBS (100 μL, 2.5 mg/mL). At 1 h after injection, the liver was irradiated using US (1 W/cm2, 10 min, 1 MHz). Subsequently, US imaging (40 MHz) of the liver imaging was performed. The corresponding echo intensity values of the ROI were quantitatively analyzed using ImageJ software.
For in vitro US imaging, the effect of ultrasound intensity on ultrasound imaging of SonoBacteriaBot has been studied, DOX-PFP-PLGA@EcM (1 mL, 1.25 mg/mL) placed in a gel model, were exposed to US irradiation with different intensities (1–3 W/cm2, 1 min, 1 MHz), and then captured US imaging (MI = 0.7, 7.5 MHz). To evaluate the effect of ultrasound treatment time on the imaging effect of SonoBacteriaBot in vitro. DOX-PFP-PLGA@EcM was subjected to ultrasonic irradiation of different durations (0–10 min, 1 W/cm2, 1 MHz), and captured US imaging. Then, Imaging of DOX-PFP-PLGA@EcM after phase transformation by ultrasound treatment under an ultrasound intensity of 1 W/cm2 for 2 min, was observed continuously (0–10 min). Ultrasound images were acquired at different times to evaluate the duration of the imaging signal.
For in vivo US imaging, mice were intravenously injected with DOX-PFP-PLGA @EcM in PBS (100 μL, 2.5 mg/mL). At 1 h after injection, the liver was irradiated using US (1 W/cm2, 10 min, 1 MHz). Subsequently, US imaging (40 MHz) of the liver imaging was performed. The corresponding echo intensity values of the ROI were quantitatively analyzed using ImageJ software.
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