β-Eudesmol and atractylodin, in their pure form (99.99%), were bought from Wako
Pure Chemical Industries Ltd (Osaka, Japan). Solvents used for dissolving
β-Eudesmol and atractylodin were ethanol (EtOH) and dimethyl sulfoxide (DMSO),
respectively. Preparation of different concentrations of the test compounds was
done by diluting the stock solutions in embryo medium (E3). The highest
concentrations of EtOH and DMSO in the final test solutions were 1.11% and 0.6%,
respectively, not exceeding the lowest permitted concentrations that cause
phenotypic and molecular level defects in the zebrafish embryos.9 –11 (link, link)
Pure Chemical Industries Ltd (Osaka, Japan). Solvents used for dissolving
β-Eudesmol and atractylodin were ethanol (EtOH) and dimethyl sulfoxide (DMSO),
respectively. Preparation of different concentrations of the test compounds was
done by diluting the stock solutions in embryo medium (E3). The highest
concentrations of EtOH and DMSO in the final test solutions were 1.11% and 0.6%,
respectively, not exceeding the lowest permitted concentrations that cause
phenotypic and molecular level defects in the zebrafish embryos.9 –
