Inflammatory HT29 Epithelial Cell Model

HT29 human intestinal epithelial cells were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in DMEM (Gibco) with 10% foetal bovine serum (04-001-1ACS, Biological Industries, Kibbutz Beit-Haemek, Israel) at 37 °C in the presence of 5% CO₂. TNFα (10 ng/mL, Z01001, GenScript Biotech, Nanjing, China) was used in cell culture with HT29 cells for 12 h to mimic an inflammatory background [30 (link)].

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Zhang J., Wang W., Zhu S, & Chen Y. (2021). Increased SERPINA3 Level Is Associated with Ulcerative Colitis. Diagnostics, 11(12), 2371.

Publication 2021

DMEM 04-001-1ACS

Biological Cell culture Cells Chinese Epithelial cells Foetal bovine serum Ht29 cells Human Inflammatory Intestinal Tnfα

Corresponding Organization : Jiangnan University

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Variable analysis

independent variables

TNFα (10 ng/mL, Z01001, GenScript Biotech, Nanjing, China)

dependent variables

Cellular response to TNFα treatment

control variables

HT29 human intestinal epithelial cells
DMEM (Gibco) with 10% foetal bovine serum (04-001-1ACS, Biological Industries, Kibbutz Beit-Haemek, Israel)
Incubation at 37 °C in the presence of 5% CO2

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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