Comparative Genomics of P. insidiosum Strains

The next-generation-sequencing-derived genomes of 10 P. insidiosum strains isolated from humans, animals, and the environment of different locations around the world, as well as 5 other Pythium species (i.e., P. irregulare strain DAOM BR486, P. ultimum strain DAOM BR144, P. iwayamai strain DAOM BR242034, P. aphanidermatum strain DAOM BR444, and P. arrhenomanes strain ATCC 12531) from the public repository, were recruited and used in this study (Table 1). The rDNA ITS-based genotype (i.e., clade I, II, or III) was assigned to all P. insidiosum strains. The resulting contig sequences were subjected to gene prediction using the MAKER2 pipeline [32 (link)]. All predicted protein sequences were annotated by comparing them to the NCBI non-redundant database using BLASTP [33 (link)].