Confocal Imaging of Nematode Specimens

Confocal images were acquired with Zeiss LSM 700 Upright confocal microscope (Carl Zeiss AG) under non-saturating exposure conditions. The worms were prepared for imaging as described16 (link). Briefly, nematodes were immobilized with 7.5 mM solution of tetramisole hydrochloride (Sigma-Aldrich) in M9 and mounted on 6% agarose pads on glass slides. Image processing was performed with the Fiji software (http://imagej.nih.gov/ij; version 1.47b). Each experiment was repeated at least once.

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Katsyuba E., Mottis A., Zietak M., De Franco F., van der Velpen V., Gariani K., Ryu D., Cialabrini L., Matilainen O., Liscio P., Giacchè N., Stokar-Regenscheit N., Legouis D., de Seigneux S., Ivanisevic J., Raffaelli N., Schoonjans K., Pellicciari R, & Auwerx J. (2018). De novo NAD+ synthesis enhances mitochondrial function and improves health. Nature, 563(7731), 354-359.

Publication 2018

Zeiss LSM 700 Upright confocal microscope tetramisole hydrochloride

Agarose Confocal microscope Nematodes Tetramisole Worms

Corresponding Organization :

Other organizations : École Polytechnique Fédérale de Lausanne, University of Lausanne, Marche Polytechnic University, University Hospital of Geneva, University of Geneva

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Confocal images of nematodes

control variables

Immobilization of nematodes with 7.5 mM solution of tetramisole hydrochloride in M9
Mounting nematodes on 6% agarose pads on glass slides
Non-saturating exposure conditions during confocal imaging

controls

Positive control: None mentioned
Negative control: None mentioned

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