Protocol detail

Drosophila Genetic Tools and Fly Maintenance

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GMR-Grim, UAS-p35, and Gal4 drivers including ptc-Gal4, nub-Gal4, GMR-Gal4, en-Gal4, and act-Gal4 were obtained from Bloomington Drosophila Stock Center (USA). UAS-ana3 RNAi line was obtained from VDRC (V101280). Ubq-ana3-GFP, UAS-ana3-GFP, and ana3^SH0558 flies were kindly provided by Jordan W. Raff. UAS-sona lines were generated in our laboratory (Kim et al., 2016 (link)). Flies were incubated at 25°C unless otherwise indicated.

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Cho D.G., Lee S.S, & Cho K.O. (2021). Anastral Spindle 3/Rotatin Stabilizes Sol narae and Promotes Cell Survival in Drosophila melanogaster. Molecules and Cells, 44(1), 13-25.

Publication 2021

ptc-Gal4 nub-Gal4 GMR-Gal4 en-Gal4 act-Gal4

Drosophila Flies Rnai

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Variable analysis

independent variables

GMR-Grim
UAS-p35
Gal4 drivers including ptc-Gal4, nub-Gal4, GMR-Gal4, en-Gal4, and act-Gal4
UAS-ana3 RNAi
Ubq-ana3-GFP
UAS-ana3-GFP
ana3^SH0558
UAS-sona

dependent variables

Not explicitly mentioned

control variables

Temperature (25°C)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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