Synthesis and Characterization of NDBP-5.5

Peptide NDBP-5.5 (UniProt P0C8W1) was identified as previously described (Schwartz et al., 2007 (link)) and was synthesized by C-terminal amidation using FastBio LTDA (Ribeirão Preto, SP, Brazil). NDBP-5.5 presented >95% of purity. The molecular mass and sequence of the synthetic NDBP5.5 were confirmed by MALDI-TOF/TOF MS (UltraFlex III, BrukerDaltonics, Germany) and LIFTTM (MS/MS) as previously described (Guilhelmelli et al., 2016 (link)).

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Trentini M.M., das Neves R.C., Santos B.D., DaSilva R.A., de Souza A.C., Mortari M.R., Schwartz E.F., Kipnis A, & Junqueira-Kipnis A.P. (2017). Non-disulfide-Bridge Peptide 5.5 from the Scorpion Hadrurus gertschi Inhibits the Growth of Mycobacterium abscessus subsp. massiliense. Frontiers in Microbiology, 8, 273.

Publication 2017

UltraFlex III

Ms maldi Ms ms Peptide

Corresponding Organization : Universidade Federal de Goiás

Other organizations : Universidade de Brasília

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Variable analysis

independent variables

Peptide NDBP-5.5 (UniProt P0C8W1)

dependent variables

Molecular mass of the synthetic NDBP-5.5
Sequence of the synthetic NDBP-5.5

control variables

Purity of the synthetic NDBP-5.5 (>95%)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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