The standard amplex red assay has been used as previously described [14 (link)] using putrescine as a substrate and native ´fibroblast derived LOX, which was prepared by collecting the supernatant of IMR90 cells, followed by a size filtration > 10 kDa for concentration and < 50 kDa to separate from LOXL2, or recombinant LOXL2 as enzyme (R&D Systems).
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