The identification of lactic acid bacteria was done by phenotypic analysis using the kit API50CHL (BioMérieux, Marcy-l'Etoile, France) according Saeed et al.(2009) and Lu et al. (2008) .The API 50CHL system allows the species identification of LAB according to the biochemical profile of carbohydrate fermentation. Readings were taken after 24 and 48 hours of incubation at 30°C. The identification of yeasts was performed using the kit API 20CAux (BioMérieux, Marcy-l'Etoile, France) according Saeed et al.(2009) and the readings were made after 48 and72 hours of incubation at 30 °C.
Protocol detail
Identification of Lactic Acid Bacteria and Yeasts
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Corresponding Organization :
Other organizations : Instituto Federal de Educação, Ciência e Tecnologia de Santa Catarina, Universidade Federal de Santa Catarina
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Variable analysis
independent variables
- Identification method for lactic acid bacteria: API50CHL kit
- Identification method for yeasts: API 20CAux kit
- Biochemical profile of carbohydrate fermentation for lactic acid bacteria
- Identification of lactic acid bacteria and yeasts
- Incubation temperature at 30°C
- Incubation time for lactic acid bacteria: 24 and 48 hours
- Incubation time for yeasts: 48 and 72 hours
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
The API 50CHL system is used to identify LAB species based on their carbohydrate fermentation profile (Input protocol, Protocol 1, Protocol 3).
Readings for the API 50CHL system are taken after 24 and 48 hours of incubation at 30°C (Input protocol).
The API 20CAux kit is used to identify yeast species (Input protocol).
Readings for the API 20CAux kit are taken after 48 and 72 hours of incubation at 30°C (Input protocol).
The API50CHL and API20CAux results are analyzed using the APILAB Plus software (Protocol 1, Protocol 2).
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