Differentiation of Bone Marrow Cells

MF patients’ or normal donors’ BM LDCs were cultured in conditions favoring differentiation to either fibrocytes or MSCs, as previously described [6 (link), 21 (link), 52 (link)]. To differentiate LDCs to fibrocytes BM LDCs were cultured in StemSpan serum-free medium (Stemcell Technologies, Vancouver, BC, Canada) supplemented with modified eagle medium (MEM), non-essential amino acid, insulin-transferrin-selenium, HEPES, glutamine, sodium pyruvate, penicillin, and streptomycin solutions (Sigma-Aldrich). To grow MSCs, BM LDCs were cultured in α-MEM supplemented with 20% FBS (Invitrogen, Waltham, MA, USA). All LDC-derived cultures were grown in Nunc Lab-Tek II CC2 chamber slides (Thermo Scientific, Waltham, MA, USA) at 37 °C in humidified atmosphere supplemented with 5% CO₂.