Targeted Somatic Mutation Detection in CTCs

For the targeted detection of somatic mutations in CTCs and tissue we used the iPLEX HS technology in conjunction with the MALDI-TOF based MassARRAY System (Agena Bioscience, San Diego, California). Here we used the Lung Panel covering 70 mutations in five key oncogenes (BRAF, EGFR, ERBB2, KRAS and PIK3CA) implicated in disease progression and therapy response. A mutation signal produced using iPLEX HS chemistry can be reliably detected by the MassARRAY System at about 1% VAF^{23 (link)}.

Free full text: Click here

Scharpenseel H., Hanssen A., Loges S., Mohme M., Bernreuther C., Peine S., Lamszus K., Goy Y., Petersen C., Westphal M., Glatzel M., Riethdorf S., Pantel K, & Wikman H. (2019). EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients. Scientific Reports, 9, 7406.

Publication 2019

MassARRAY System

Braf Disease progression Egfr Erbb2 Iplex Kras Lung Maldi Mutation Oncogenes Pik3ca Somatic Targeted mutations Therapy Tissue

Corresponding Organization :

Other organizations : Universität Hamburg, University Medical Center Hamburg-Eppendorf

Top 5 similar protocols

Variable analysis

independent variables

Targeted detection of somatic mutations in CTCs and tissue

dependent variables

Mutation signal produced using iPLEX HS chemistry
Ability to reliably detect mutation signal at about 1% VAF

control variables

Use of the MALDI-TOF based MassARRAY System (Agena Bioscience, San Diego, California)
Use of the Lung Panel covering 70 mutations in five key oncogenes (BRAF, EGFR, ERBB2, KRAS and PIK3CA)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!